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【英文标准名称】:StandardPracticeforTissueCryosectionAnalysiswithSIMS
【原文标准名称】:用次级离子质谱法(SIMS)进行组织低温截面分析的标准实施规程
【标准号】:ASTME1880-2006
【标准状态】:现行
【国别】:
【发布日期】:2006
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E42.06
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:生物工艺学;低温学;成象;质谱法;微生物分析;光学;组织
【英文主题词】:SIMS
【摘要】:PressingcryosectionsflatontoaconductingsubstratehasbeenoneofthemostchallengingproblemsinSIMSanalysisofcryogenicallypreparedtissuespecimens.Frozencryosectionsoftencurlorpeeloff,orboth,fromthesubstrateduringfreeze-drying.ThecurlingofcryosectionsresultsinanunevensamplesurfaceforSIMSanalysis.Furthermore,iffreeze-driedcryosectionsarenotattachedtightlytothesubstrate,theimpactoftheprimaryionbeammayresultinfurthercurlingandevendislodgingofthecryosectionfromthesubstrate.TheseproblemsrenderSIMSanalysisdifficult,frustratingandtimeconsuming.Theuseofindiumasasubstrateforpressingcryosectionsflathasprovidedapracticalapproachforanalyzingcryogenicallypreparedtissuespecimens.(1)TheproceduredescribedhereinhasbeensuccessfullyusedforSIMSimagingofcalciumandmagnesiumtransportandlocalizationofanticancerdrugsinanimalmodels(2,3,4,5)Theproceduredescribedhereisamenabletosofttissuesofbothanimalandplantorigin.1.1ThispracticeprovidestheSecondaryIonMassSpectrometry(SIMS)analystwithamethodforanalyzingtissuecryosectionsintheimagingmodeoftheinstrument.Thispracticeissuitableforfrozen-freeze-driedandfrozen-hydratedcryosectionanalysis.1.2Thispracticedoesnotdescribemethodsforoptimalfreezingofthespecimenforimmobilizingdiffusiblechemicalspeciesintheirnativeintracellularsites.1.3Thispracticedoesnotdescribemethodsforobtainingcryosectionsfromafrozenspecimen.1.4Thispracticeisnotsuitableforanyplasticembeddedtissues.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:A40
【国际标准分类号】:17_180_01
【页数】:2P.;A4
【正文语种】:


Product Code:SAE J1403
Title:Vacuum Brake Hose
Issuing Committee:Automotive Brake And Steering Hose Standards Comm
Scope:The vacuum brake hose is intended for use in the power braking systems of vehicles or as connections on transmission lines in combinations of vehicles or systems thereof. For the purposes of clearly identifying hose classification and for specification simplification, vacuum brake hose is divided into two types: heavy-wall Type H, and light-wall Type L.